Recent Publications

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  1. Howlader DR, Das S, Lu T, Hu G, Varisco DJ, Dietz ZK, Walton SP, Ratnakaram SSK, Gardner FM, Ernst RK, Picking WD, Picking WL. "Effect of Two Unique Nanoparticle Formulations on the Efficacy of a Broadly Protective Vaccine Against Pseudomonas Aeruginosa." (2021).  Front Pharmacol. 12:706157. 
  2.  Tachiyama S, Skaar R, Chang Y, Carroll BL, Muthuramalingam M, Whittier SK, Barta ML, Picking WL, Liu J, Picking WD. "Composition and Biophysical Properties of the Sorting Platform Pods in the Shigella Type III Secretion System." (2021). Front Cell Infect Microbiol. 11:682635. 
  3.  Muthuramalingam M, Whittier SK, Picking WL, Picking WD. (2021). "The Shigella Type III Secretion System: An Overview from Top to Bottom." Microorganisms. 9(2):451. 
  4.  Zacharia A, Harberts E, Valencia SM, Myers B, Sanders C, Jain A, Larson NR, Middaugh CR, Picking WD, Difilippantonio S, Kirnbauer R, Roden RB, Pinto LA, Shoemaker RH, Ernst RK, Marshall JD. (2021). Optimization of RG1-VLP vaccine performance in mice with novel TLR4 agonists. Vaccine. 39(2):292-302. 
  5.  Das S, Howlader DR, Zheng Q, Ratnakaram SSK, Whittier SK, Lu T, Keith JD, Picking WD, Birket SE, Picking WL. (2020). "Development of a Broadly Protective, Self-Adjuvanting Subunit Vaccine to Prevent Infections by Pseudomonas aeruginosa." Front Immunol. 11:583008. 
  6.  Muthuramalingam M, Whittier SK, Lovell S, Battaile KP, Tachiyama S, Johnson DK, Picking WL, Picking WD. (2020). "The Structures of SctK and SctD from Pseudomonas aeruginosa Reveal the Interface of the Type III Secretion System Basal Body and Sorting Platform." J Mol Biol. 432(24):166693. 
  7.  Montoya NA, Barr KE, Morales SV, Umana JE, Ny C, Roth RE, Reyes EJ, Kirchhoff BC, Hartman ER, Higgins LL, Nichol KM, Morais ARC, Allgeier AM, Gao P, Picking WD, Corbin DR, Shiflett MB. (2020). "Protein Stabilization and Delivery: A Case Study of Invasion Plasmid Antigen D Adsorbed on Porous Silica." Langmuir. 36(47):14276-14287.
  8.  Jain A, Hu G, Kumar Ratnakaram SS, Johnson DK, Picking WD, Picking WL, Middaugh CR. (Epub 2020, pub 2021). "Preformulation Characterization and the Effect of Ionic Excipients on the Stability of a Novel DB Fusion Protein." J Pharm Sci. 110(1):108-123. 
  9. Montoya N, Barr K, Kirchhoff B, Reyes E, Umana J, Nichol K, Hartman E, Picking W, Gao FP, Corbin DR, Shiflett MB. (2020). "Development of Silica-Immobilized Vaccines for Improving Thermo-Tolerance and Shelf-Life." Kans J Med.  13(Suppl 2): 6-9. 
  10. Picking WD, Barta ML. (2020). "The Tip Complex: From Host Cell Sensing to Translocon Formation." Curr Top Microbiol Immunol. 427: 173-199
  11. Tachiyama S, Chang Y, Muthuramalingam M, Hu B, Barta ML, Picking WL, Liu J, Picking WD (2019). "The cytoplasmic domain of MxiG interacts with MxiK and directs assembly of the sorting platform in the Shigella type III secretion system." J Biol Chem.  294(50): 19184-19196.
  12. Muthuramalingam M, Middaugh CR, Picking WD. (2019). "The cytoplasmic portion of the T3SS inner membrane ring components sort into distinct families based on biophysical properties. "Biochim Biophys Acta Proteins Proteom. 1867(9): 787-793.
  13. Arizmendi O, Kumar P, Zheng Q, Stewart JP, Picking WD, Picking W, Martinez-Becerra FJ. (2019). "Vaccination With Mouse Dendritic Cells Loaded With an IpaD-IpaB Fusion Provides Protection Against Shigellosis." Front Immunol. 10: 192. 
  14. Heine SJ, Franco-Mahecha OL, Sears KT, Drachenberg CB, van Roosmalen ML, Leenhouts K, Picking WL, Pasetti MF. (2019). "A Combined YopB and LcrV Subunit Vaccine Elicits Protective Immunity against Yersinia Infection in Adult and Infant Mice." J Immunol. 202(7): 2005-2016.
  15. Barta ML, Tachiyama S, Muthuramalingam M, Arizmendi O, Villanueva CE, Ramyar KX, Geisbrecht BV, Lovell S, Battaile KP, Picking WL, Picking WD. (2018). "Using disruptive insertional mutagenesis to identify the in situ structure-function landscape of the Shigella translocator protein IpaB." Protein Sci.  Aug. 27(8): 1392-1406.
  16. Martinez-Becerra FJ, Kumar P, Vishwakarma V, Kim JH, Arizmendi O, Middaugh CR, Picking WD, Picking WL (2018). "Characterization and Protective Efficacy of Type III Secretion Proteins as a Broadly Protective Subunit Vaccine against Salmonella enterica Serotypes." Infect Immun  86(3).
  17. Wei Y, Wahome N, Kumar P, Whitaker N, Picking WL, Middaugh CR (2018; Epub 2017). "Effect of Phosphate Ion on the Structure of Lumazine Synthase, an Antigen Presentation System from Bacillus anthracis." J Pharm Sci  107(3): 814-823.
  18. Salerno-Gonçalves R, Tettelin H, Lou D, Steiner S, Rezwanul T, Guo Q, Picking WD, Nene V, Sztein MB (2017). "Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells." PLoS Negl Trop Dis 11(9): e0005912.
  19. Barta ML, Shearer JP, Arizmendi O, Tremblay JM, Mehzabeen N, Zheng Q, Battaile KP, Lovell S, Tzipori S, Picking WD, Shoemaker CB, Picking WL (2017). "Single-domain antibodies pinpoint potential targets within Shigella invasion plasmid antigen D of the needle tip complex for inhibition of type III secretion." J Biol Chem  292 (40): 16677-16687.
  20. Wei Y, Wahome N, VanSlyke G, Whitaker N, Kumar P, Barta ML, Picking WL, Volkin DB, Mantis NJ, Middaugh CR (2017).  "Evaluation of Lumazine Synthase from Bacillus anthracis as a Presentation Platform for Polyvalent Antigen Display."  Protein Sci (10): 2059-2072.
  21. Dumas EK, Garman L, Cuthbertson H, Charlton S, Hallis B, Engler RJM, Choudhari S, Picking WD, James JA, Farris AD (2017). "Lethal factor antibodies contribute to lethal toxin neutralization in recipients of anthrax vaccine precipitated."  Vaccine  35(26): 3416-3422.
  22. Kupgan G, Choudhari SP, Flynn NH, Nigatu A, Vupputuri S, Picking WL, Picking WD, Ramsey JD.(2017).  "Identification of Excipients for Stabilizing Fiberless Adenovirus as Biopharmaceuticals."  J Pharm Sci  106(7): 1764-1771.
  23. Caruso G, Fresta CG, Martinez-Becerra F, Antonio L, Johnson RT, de Campos RPS, Siegel JM, Wijesinghe MB, Lazzarino G, Lunte SM (2017). "Carnosine modulates nitric oxide in stimulated murine RAW 264.7 macrophages."  Mol Cell Biochem  431(1-2): 197-210.
  24. Hays MP, Kumar A, Martinez-Becerra FJ, Hardwidge PR. (2016). "Immunization with the MipA, Skp, or ETEC_2479 Antigens Confers Protection against Enterotoxigenic E. coli Strains Expressing Different Colonization Factors in a Mouse Pulmonary Challenge Model."  Front Cell Infect Microbiol  6: 181.
  25. Picking WL, Picking WD. (2016). "The Many Faces of IpaB." Front Cell Infect Microbiol 6: 12.
  26. Hartwell BL, Martinez-Becerra FJ, Chen J, Shinogle H, Sarnowski M, Moore DS, Berkland C. (2016). "Antigen-Specific Binding of Multivalent Soluble Antigen Arrays Induces Receptor Clustering and Impedes B Cell Receptor Mediated Signaling." Biomacromolecules 17(3): 710-22.
  27. Chen X, Choudhari SP, Kumar P, Toth RT 4th, Kim JH, Van Roosmalen ML, Leenhouts K, Middaugh CR, Picking WL, Picking WD. (2015). "Biophysical Characterization of the Type III Secretion System Translocator Proteins and the Translocator Proteins Attached to Bacterium-Like Particles." J Pharm Sci 104(12): 4065-73.
  28. Heine SJ, Franco-Mahecha OL, Chen X, Choudhari S, Blackwelder WC, van Roosmalen ML, Leenhouts K, Picking WL, Pasetti MF. (2015). "Shigella IpaB and IpaD displayed on L. lactis bacterium-like particles induce protective immunity in adult and infant mice." Immunol Cell Biol 93(7): 641-652.
  29. Chen, X., Choudhari, S. P., Martinez-Becerra, F. J., Kim, J. H., Dickenson, N. E., Toth, R. T. th, Joshi, S. B., Greenwood, J. C., 2nd, Clements, J. D., Picking, W. D., Middaugh, C. R., Picking, W. L. (2015). "Impact of detergent on biophysical properties and immune response of the IpaDB fusion protein, a candidate subunit vaccine against Shigella species." Infect Immun 83(1): 292-299.
  30. Choudhari, S. P., Chen, X., Kim, J. H., Van Roosmalen, M. L., Greenwood, J. C., 2nd, Joshi, S. B., Picking, W. D., Leenhouts, K., Middaugh, C. R., Picking, W. L. (2015). "Biophysical Characterization of the Type III Secretion Tip Proteins and the Tip Proteins Attached to Bacterium-Like Particles." J Pharm Sci 104(2): 424-432.
  31. Heine, S. J., Diaz-McNair, J., Andar, A. U., Drachenberg, C. B., van de Verg, L., Walker, R., Picking, W. L., Pasetti, M. F. (2014). "Intradermal delivery of Shigella IpaB and IpaD type III secretion proteins: kinetics of cell recruitment and antigen uptake, mucosal and systemic immunity, and protection across serotypes." J Immunol 192(4): 1630-1640.
  32. Lee, S., Picking, W. L., Tzipori, S. (2014). "The immune response of two microbial antigens delivered intradermally, sublingually, or the combination thereof." Microbes Infect 16(9): 796-803.
  33. Snider, T. A., Gull, T., Jackson, T. A., Martinez-Becerra, F. J., Picking, D. R., Picking, W. D., Picking, W. L. (2014). "Experimental salmonellosis challenge model in older calves." Vet Microbiol 170(1-2): 65-72.
  34. Choudhari, S. P., Pendleton, K. P., Ramsey, J. D., Blanchard, T. G., Picking, W. D. (2013). "A systematic approach toward stabilization of CagL, a protein antigen from Helicobacter pylori that is a candidate subunit vaccine." J Pharm Sci 102(8): 2508-2519.
  35. Heine, S. J., Diaz-McNair, J., Martinez-Becerra, F. J., Choudhari, S. P., Clements, J. D., Picking, W. L., Pasetti, M. F (2013). "Evaluation of immunogenicity and protective efficacy of orally delivered Shigella type III secretion system proteins IpaB and IpaD." Vaccine 31(28): 2919-2929.
  36. Martinez-Becerra, F. J., Chen, X., Dickenson, N. E., Choudhari, S. P., Harrison, K., Clements, J. D., Picking, W. D., Van De Verg, L. L., Walker, R. I., Picking, W. L. (2013). "Characterization of a novel fusion protein from IpaB and IpaD of Shigella spp. and its potential as a pan-Shigella vaccine." Infect Immun 81(12): 4470-4477.
  37. Martinez-Becerra, F. J., Scobey, M., Harrison, K., Choudhari, S. P., Quick, A. M., Joshi, S. B., Middaugh, C. R., Picking, W. L. (2013). "Parenteral immunization with IpaB/IpaD protects mice against lethal pulmonary infection by Shigella." Vaccine 31(24): 2667-2672.
  38. Wahid, R., Simon, J. K., Picking, W. L., Kotloff, K. L., Levine, M. M., Sztein, M. B. (2013). "Shigella antigen-specific B memory cells are associated with decreased disease severity in subjects challenged with wild-type Shigella flexneri 2a." Clin Immunol 148(1): 35-43.
  39. Barta, M. L., Dickenson, N. E., Patil, M., Keightley, A., Wyckoff, G. J., Picking, W. D., Picking, W. L., Geisbrecht, B. V. (2012). "The structures of coiled-coil domains from type III secretion system translocators reveal homology to pore-forming toxins." J Mol Biol 417(5): 395-405.
  40. Martinez-Becerra, F., Castillo-Rojas, G., Ponce de Leon, S., Lopez-Vidal, Y. (2012). "IgG subclasses against Helicobacter pylori isolates: an important tool for disease characterization." Scand J Immunol 76(1): 26-32.
  41. Martinez-Becerra, F. J., Kissmann, J. M., Diaz-McNair, J., Choudhari, S. P., Quick, A. M., Mellado-Sanchez, G., Clements, J. D., Pasetti, M. F., Picking, W. L. (2012). "Broadly protective Shigella vaccine based on type III secretion apparatus proteins." Infect Immun 80(3): 1222-1231.
  42. Simon, J. K., Maciel, M., Jr., Weld, E. D., Wahid, R., Pasetti, M. F., Picking, W. L., Kotloff, K. L., Levine, M. M., Sztein, M. B. (2011). "Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates." Clin Immunol 139(2): 185-192.
  43. Barrett, B. S., Markham, A. P., Esfandiary, R., Picking, W. L., Picking, W. D., Joshi, S. B., Middaugh, C. R. (2010). "Formulation and immunogenicity studies of type III secretion system needle antigens as vaccine candidates." J Pharm Sci 99(11): 4488-4496.
  44. Markham, A. P., Barrett, B. S., Esfandiary, R., Picking, W. L., Picking, W. D., Joshi, S. B., Middaugh, C. R. (2010). "Formulation and immunogenicity of a potential multivalent type III secretion system-based protein vaccine." J Pharm Sci 99(11): 4497-4509.
  45. Ramirez, K., Ditamo, Y., Rodriguez, L., Picking, W. L., van Roosmalen, M. L., Leenhouts, K., Pasetti, M. F. (2010). "Neonatal mucosal immunization with a non-living, non-genetically modified Lactococcus lactis vaccine carrier induces systemic and local Th1-type immunity and protects against lethal bacterial infection." Mucosal Immunol 3(2): 159-171.

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